Objective To explore the effects of urine sediment indicators on the detection level of Alzheimer-associated neuronal thread protein （AD7c-NTP）. Methods A total of 314 healthy physical examination subjects in Affiliated Hospital of Guilin Medical University from July 2021 to October 2021 were included. Urine samples were collected for AD7c-NTP enzyme linked immunosorbent assay （ELISA） detection. Age， gender， and urine sediment detection indicators were collected and the effects of urinary sediment indexes on AD7c-NTP levels were analyzed. Results The levels of AD7c-NTP in the urine of male subjects with different levels of red blood cells， white blood cells， epithelial cells， small round epithelial cells， casts， yeast like bacteria and crystals showed no statistically significant difference （P ＞ 0.05）； In males， the AD7c-NTP levels in low conductivity group ［0.68 （0.48，1.40） ng/ml］， medium conductivity group ［0.50（0.32，1.06） ng/ml］ and high conductivity group ［0.34（0.31，0.51） ng/ml］ showed a significant decreasing trend. The difference is statistically significant （Z=-3.823，P ＜ 0.05）. There is no statistically significant difference in AD7c-NTP levels in urine of female subjects with different levels of red blood cells， white blood cells， epithelial cells， small round epithelial cells， tube types， yeast like bacteria and electrical conductivity （P＞ 0.05）； The AD7cNTP levels in urine of female subjects with high crystal level ［0.51（0.33，0.62） ng/ml］ was lower than that of female subjects with low crystal level ［0.65（0.42，1.18） ng/ml］. The difference is statistically significant （Z= -2.331， P＜ 0.05）. Conclusions Among all the urine sediment indicators， the conductivity in male and the crystal in female may affect the detection level of AD7c-NTP.