Objective To explore the clinical effect of mesenchymal stem cells （MSCs） transplantation on urinary disorders of spinal cord injured patients through urodynamic test. Methods 20 patients with sequela of thoracolumbar spinal cord injury were enrolled to receive MSCs transplantation. Urodynamic test and ASIA scores were carried out before and half a year after the transplantation. Results Significant improvements including function of urethral sphincter and bladder compliance were observed in 60% patients after the transplantation. Conclusions MSCs transplantation could significantly improve urination disorders, promote the recovery of urethral sphincter and bladder functions and improve life quality for patients with thoracolumbar spinal cord injury.