Post-stroke depression（ PSD） is a common clinical complication occurring after ischemic and hemorrhagic cerebral infarction. About 1/3 of stroke patients develop PSD， and its incidence rate is 4 to 10 times higher than that in the general population. In recent years， efforts have been made to identify biomarkers related to PSD. Despite the lack of evidence supporting the use of a particular biomarker for PSD， several changes have been observed in oxidative stress， hypothalamic-pituitary-adrenal axis dysfunction， neurotransmitter alterations， neurotrophic factor changes， gut microbiota imbalance， and genetic susceptibility. In addition， neuroimaging studies can detect early subtle structural brain damage in patients with PSD， and the integration with biological biomarkers may enhance the diagnostic and predictive accuracy for this condition. This article reviews the biological markers and imaging examinations related to PSD， aiming to understand the pathophysiology， prevention， and treatment of PSD and provide assistance.