Objective To explore the influence of neural stem cells（NSCs）supernatants on bone mar-row derived-macrophage，in order to provide new ideas for therapy. Methods Mouse bone marrow macrophage were treated with NSCs supernatant for 3 d. SRB assay was used to detect the proliferation of macrophage. Thephagocytosis of macrophage were detected by oil red staining after adding myelin for 24 h. Meanwhile，the expression of TNF-α，IL-6 and IL-10 in the macrophage supernatant were detected by ELISA kit. Results Macrophagewere obtained after bone marrow-derived cells cultured in the medium containing L929 supernatant for 7 d. Re-spectively，macrophage proliferation rate of NSCs medium group，NSCs supernatant and ESC-NSCs superatant group was（100±1.2）%，（135±2.1）% and （132±3.4）%. Oil red percentage of positive cells was （50.7±4.5）%，（92.2±3.7）% and （89.5±4.3）% respectively. Compared to NSCs medium group，the levels of IL-6 and IL-10 in macrophage treated with NSCs and ESC-NSCs supernatant significantly increased，and the expression of TNF-α significantly decreased （P＜ 0.05）. Conclusions Both primary NSCs supernatant and ESC-derivedNSCs supernatant could promote the proliferation and phagocytosis of bone marrow macrophage.